Expression patterns of Doppel in differential ovine PRNP genotypes: quantification using real-time RT-PCR
نویسندگان
چکیده
منابع مشابه
Tissue-specific expression pattern of bovine prion gene: quantification using real-time RT-PCR.
In recent studies PrP mRNA was determined mostly by in situ hybridisation or Northern Blot analysis--methods not suitable for absolute quantification of mRNA copy numbers. Herein we report on bovine prion mRNA quantification using calibrated highly sensitive externally standardized real-time RT-PCR with LightCycler instrument. Total RNA was isolated from nine different regions of the CNS and se...
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ژورنال
عنوان ژورنال: Genetics and Molecular Research
سال: 2015
ISSN: 1676-5680
DOI: 10.4238/2015.october.9.3